Application
| WB, IHC, E, IP, RIA |
|---|---|
| Primary Accession | P04637 |
| Other Accession | NP_000537.3 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human p53 Monoclonal IgG1 |
| Target/Specificity | Detects ~53kDa. |
| Other Names | P53 Antibody, LFS1 Antibody, TRP53 Antibody, FLJ92943 Antibody, TP53 Antibody, p53 Tumor Suppressor Antibody, Phosphoprotein p53 Antibody, TP53 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor protein p53 Antibody |
| Clone Names | Pab 1801 |
| Immunogen | Fusion protein (Human), amino acids 46-53 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm | Nucleus | PML body | Endoplasmic Reticulum |
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Provided below are standard protocols that you may find useful for product applications.
Background
The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
References
1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9.
2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics.
3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774.
4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8.
5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.