ELISA. Western Blot. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid (Wilson, E. et al. (2007) J.Immunol.Methods. 322: 137-42. Useful in functional studies.
Reactivity
Rat
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Activated, Lewis Rat derived microglial cells.
Specificity
This antibody recognises PECAM-1 (CD31). Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
Buffer
PBS without preservatives or stabilizers State: Low Endotoxin State: Liquid purified IgG fraction
Concentration
lot specific
Purification
Affinity Chromatography on Protein G
Conjugation
Unconjugated
Storage
Store the antibody undiluted at -20°C. Avoid repeated freezing and thawing.
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). CD31 is a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
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