ASM10533-A633-100 | PI 3 Kinase Class 3 antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533). Tissue: C2C12 Cells (Mouse Myoblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.
Western blot analysis of Rat, Mouse Liver cell lysates showing detection of ~101.5 kDa PI 3 Kinase Class 3 protein using Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Lane 3: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PI 3 Kinase Class 3 Polyclonal Antibody (ASM10533) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~101.5 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q8NEB9
Other Accession	NP_002638.2
Host	Rabbit
Reactivity	Mouse, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human PI 3 Kinase Class 3 Polyclonal
Target/Specificity	Detects ~101.5 kDa.
Other Names	PI3 kinase type 3 Antibody, PI 3 kinase type 3 Antibody, hVps34 Antibody, Phosphatidylinositol 3-kinase p100 subunit Antibody, Phosphatidylinositol 3 kinase p100 subunit Antibody, PI3K type 3 Antibody, PI3-kinase type 3 Antibody, EC:2.7.1.137 Antibody, PK3C3_HUMAN Antibody, PtdIns-3-kinase type 3 Antibody, PIK3C3 Antibody, VPS34 Antibody, Pik3c3 Antibody, Phosphoinositide 3 kinase class 3 Antibody, Phosphatidylinositol 3-kinase catalytic subunit type 3 Antibody, PtdIns 3 kinase type 3 Antibody, Phosphoinositide-3-kinase class 3 Antibody, Phosphatidylinositol 3 kinase class 3 Antibody, Vps 34 Antibody, Phosphatidylinositol 3 kinase catalytic subunit type 3 Antibody, MGC61518 Antibody, Vps34 Antibody, PI3 kinase class 3 Antibody
Immunogen	Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-661 was sufficient for detection of PI 3 Kinase Class 3 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Midbody \| Late Endosome \| Cytoplasmic Vesicle \| Autophagosome

Research Areas

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