PS100062 | pMirTarget 3' UTR Assay Vector Clinisciences

pMirTarget 3' UTR Assay Vector

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PS100062

pMirTarget, the cloning vector for all 3' UTR clones for miRNA target validation, 10ug

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Datasheet SDS Vector Sequence

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Specifications
Product Data
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Features	pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3' UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity. High sensitivity of Luciferase assay due to IRES-driven luciferase cassette RFP as a reporter for transfection monitoring and normalization Neomycin selection marker for stable cell establishment Compatible with Precision shuttling system Can serve as a control for target validation experiments. 3' UTR Clones for MicroRNA target validation LUC_F Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites

Specifications

Product Data

E. coli Selection

Kanamycin (25 ug/mL)

Mammalian Cell Selection

Neomycin

Features

pMirTarget is a reporter construct with firefly luciferase as a reporter. The multiple cloning sites are downstream of luciferase stop codon and allow insertion of any sequence of interest, creating a chimerical transcript. All of OriGene’s 3' UTR clones are constructed in this vector. In the presence of appropriate miRNA (external or internal), shRNA or siRNA, the RNAi machinery would interact with the 3’UTR sequence and causes reduction of the luciferase activity.

High sensitivity of Luciferase assay due to IRES-driven luciferase cassette
RFP as a reporter for transfection monitoring and normalization
Neomycin selection marker for stable cell establishment
Compatible with Precision shuttling system
Can serve as a control for target validation experiments.
3' UTR Clones for MicroRNA target validation
LUC_F Primer can be used to sequence the target sequence after cloning.

Schematic of the multiple cloning sites

pMirTarget 3' UTR Assay Vector

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pMirTarget 3' UTR Assay Vector

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