PreScission Protease

Biological Activity

PreScission Protease is a recombinantly expressed 3C protease of human rhinovirus type 14 with a GST tag in Escherichia coli that specifically recognizes the octapeptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro or the core pentapeptide sequence Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and cleaves between Gln and Gly amino acid residues. Pro and enzymatically cleaves between Gln and Gly amino acid residues, commonly used to remove Glutathione S-transferase (GST), His, or other tags from fusion proteins.

Chemical Information

Form	Solution
Storage	-20℃

References

[1] Jean-Christophe Amé et al. Methods Mol Biol. Purification of Recombinant Human PARG and Activity Assays

[2] Shuwen Zhou et al. Biophys Chem. A novel assay for the determination of PreScission protease by capillary electrophoresis

[3] Elwy H Abdelkader et al. J Biotechnol. NT*-HRV3CP: An optimized construct of human rhinovirus 14 3C protease for high-yield expression and fast affinity-tag cleavage

[4] Jianhao Wang et al. J Sep Sci. Simultaneous monitoring of quantum dots and their assembly and disassembly with PreScission protease using capillary electrophoresis with fluorescence detection

[5] Federica Volontè et al. Microb Cell Fact. Optimizing HIV-1 protease production in Escherichia coli as fusion protein