In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Background
Pyruvate kinase (PK) is an important enzyme involved in glycolysis metabolic pathway. PK catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, producing ATP and pyruvate. Lack of pyruvate kinase will cause the disease pyruvate kinase deficiency which slows down the process of glycolysis.
The Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit provides a sensitive, simple, fast and convenient way for accurate detection of PK activity in various samples (blood, tissues, and culture cells, etc.) based on colorimetric and fluorometric method. In the assay, PEP and ADP were catalyzed by PK to produce ATP and pyruvate, which is subsequently oxidized by pyruvate oxidase to generate fluorescence (Ex/Em = 535/587 nm) and color (λ = 570 nm). The increase in fluorescence intensity or color is proportional to the increase in pyruvate amount. The kit can detect 0.1 mU/mL pyruvate kinase.
Features & Properties
Features
Fast and convenient; Simple procedure; Takes approx. less than 40 minutes.