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> RayPlex Human Growth Factor Bead Array 1

RayPlex Human Growth Factor Bead Array 1

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Referentie FAH-HGF-1-192

Formaat : 192tests

Merk : RayBiotech

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Telefoonnummer : +1 850 650 7790

RayPlex Human Growth Factor Bead Array 1

This RayPlex® kit is a cytometric bead array to quantitatively measure 12 growth factors with a flow cytometer. The assay has been optimized for serum, plasma, and cell culture supernatant.

As low as

Product Description

Specifications

Size 48 tests, 96 tests, 192 tests, 480 tests
Species Human
Quantitative/Semi-Quantitative Quantitative
Number of Targets Detected 12
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support Fluorescent Microbeads
Method Of Detection Flow Cytometry
Design Principle Sandwich-based
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage 4°C

Product Features

  • Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
  • 4 hour processing time
  • 5-10x more cost-effective than ELISA
  • Compatible with most flow cytometers
  • No specialized or dedicated instrument(s) needed
  • High specificity and system reproducibility
  • Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Angiopoietin-2
  • Gene Symbol: ANGPT2
  • Gene ID: 285
  • Accession #:
  • ELISA Kit:
bFGF
  • Gene Symbol: FGF2 FGFB
  • Gene ID: 2247
  • Accession #:
  • ELISA Kit:
EGF
  • Gene Symbol: EGF
  • Gene ID: 1950
  • Accession #:
  • ELISA Kit:
GCSF
  • Gene Symbol: CSF3 C17orf33 GCSF
  • Gene ID: 1440
  • Accession #:
  • ELISA Kit:
GM-CSF
  • Gene Symbol: CSF2 GMCSF
  • Gene ID: 1437
  • Accession #:
  • ELISA Kit:
HGF
  • Gene Symbol: HGF HPTA
  • Gene ID: 3082
  • Accession #:
  • ELISA Kit:
M-CSF
  • Gene Symbol: CSF1
  • Gene ID: 1435
  • Accession #:
  • ELISA Kit:
PDGF-AA
  • Gene Symbol: PDGFA PDGF1
  • Gene ID: 5154
  • Accession #:
  • ELISA Kit:
PDGF-BB
  • Gene Symbol: PDGFB PDGF2 SIS
  • Gene ID: 5155
  • Accession #:
  • ELISA Kit:
SCF
  • Gene Symbol: KITLG MGF SCF
  • Gene ID: 4254
  • Accession #:
  • ELISA Kit:
TGF alpha
  • Gene Symbol: TGFA
  • Gene ID: 7039
  • Accession #:
  • ELISA Kit:
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 7422
  • Accession #:
  • ELISA Kit:
     

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • RayPlex Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • RayBio® Serum/Plasma Diluent
  • Lyophilized Protein Standard Mix
  • 1X Biotinylated Detection Antibody Cocktail
  • 10X Streptavidin-PE
  • Flow Cytometer Setup Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate
Other Materials Required
  • Orbital 96-well plate shaker (with ability to reach 1000 rpm)
  • Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
  • Aluminum foil or 96-well Plate Aluminum Sealers
  • Distilled water
  • 1.5 ml polypropylene microcentrifuge tubes or similar
  • Microcentrifuge
  • If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
  • Optional: 96-well round bottom plate (to prepare samples)
  • Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. !

Protocol Outline
  1. Prepare Reagents, Standards, and Samples
  2. Add RayPlex Multiplex Bead Cocktail to each well
  3. Incubate with Samples and Standards
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluorophore
  6. Analyze with a compatible flow cytometer
  7. Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.