Recombinant Human IFNγ (CHO)

Biological Activity

IFNγ (CHO) is a cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. IFNγ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFNγ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNγ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.

ED50 < 2.0 ng/ml, measured in a cytotoxicity assay using HT-29 cells, corresponding to a specific activity of > 5.0 × 10⁵ units/mg.

Chemical Information

Molecular Weight	17145.36
Formula	C761H1206N214O225S6
CAS Number	98059-61-1
Form	Lyophilized powder
Solubility (25°C)	Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.

References

[1] Matsuda A, et al. Invest Ophthalmol Vis Sci. Genetic polymorphisms in the promoter of the interferon gamma receptor 1 gene are associated with atopic cataracts.

[2] Park-Min KH, et al. Immunity. FcgammaRIII-dependent inhibition of interferon-gamma responses mediates suppressive effects of intravenous immune globulin.