Recombinant Human IFN-β（HEK 293)

Biological Activity

Expression system: HEK 293
Purity: SDS-PAGE, & GT; 95%
Endotoxin: determination of protein by gel coagulation & LT; 0.2 EU/mu g
Apparent molecular weight: ~23 kDa, sdS-PAGE under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.

Biological activity: Interferon β (IFN-β) acts via STAT1 and STAT2 and is known to up-regulate and down-regulate a variety of genes, most of which are involved in antiviral immune responses. It is a member of type I interferon, which includes IFN-α, -β, τ and -ω. IFN-β plays an important role in inducing nonspecific resistance to a wide range of viral infections. It also affects cell proliferation and modulates immune responses.

Chemical Information

Storage

Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Zhang Xiangbo, et al. Arch Microbiol. Bovine coronavirus nucleocapsid suppresses IFN-β production by inhibiting RIG-I-like receptors pathway in host cells

[2] Samira Khiar, et al. Sci Rep. Identification of a small molecule that primes the type I interferon response to cytosolic DNA

[3] Kay-Sara Sauter, et al. Vet Immunol Immunopathol. Stable transduction of bovine TLR4 and bovine MD-2 into LPS-nonresponsive cells and soluble CD14 promote the ability to respond to LPS

[4] Meenakshi Upreti, et al. Biochem Biophys Res Commun. Replacement of 198MQMDII203 of mouse IRF-1 by 197IPVEVV202 of human IRF-1 abrogates induction of IFN-beta, iNOS, and COX-2 gene expression by IRF-1