Recombinant human IL-33 protein

Cat No.
ILC0701

Size

QTY

List




Product Information

Product Type
Recombinant Protein
Expression System
E.coli
Domain
112-270aa
UniProt No.
O95760
NCBI Accession No.
NP_254274
Alternative names
Interleukin-33, Interleukin-1 family member 11, IL-1F11, Nuclear factor from high endothelial venules, NF-HEV, DVS27-related protein, DVS27, C9orf26

Product Specification

Molecular Weight
18.1 kDa (160aa) confirmed by MALDI-TOF
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.3ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Human IL-33 in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.3ng/ml.

Related Publications

    Ito R, et al. A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axis. (JCI Insight. 2018)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.