Recombinant Human TNF Protein
Referentie CRP2401-10ug
Formaat : 10ug
Merk : Cohesion Biosciences
Product Name: | Recombinant Human TNF Protein |
Cat No: | CRP2401 |
Source: | E. coli |
Reactivity: | H |
Applications: | E, WB, SDS-PAGE, MS |
*Application Key: | |
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish | |
*Species Reactivity Key: | |
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference |
Description:Recombinant Human TNF Protein is produced by E. coli expression system and the target gene encoding Gly57-Leu233 is expressed with a 6His tag at the N-terminus.Form:Lyophilized from a 0.2 μM filtered solution of 20mM PB, 100mM NaCl, pH 7.2.Gene Symbol:TNFAlternative Names:TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a Entrez Gene (Human): 7124; SwissProt (Human): P01375; Purity:Greater than 95% as determined by reducing SDS-PAGE.Chemical Structure:MGSSHHHHHH SSGLVPRGSH MGPQREEFPR DLSLISPLAQ AVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIALDirections for Use:Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Quality Control:Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.Storage/Stability:Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.