Formaat : 10 ug
Telefoonnummer : +1 850 650 7790
| Symansis' purified IL-4hcx has N-linked, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and may have O-linked oligosaccharides. Symansis' IL-4hcx consists of up to 25% carbohydrate by weight. Densitometry of protein isoforms visualised by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 1. Kay NE & Pittner BT (2003) Leuk Lymphoma 44: 897-903. |  |  |
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