VGP1120 | SARS-CoV-2 Spike Peptide (KNLNESLIDLQELGK) Clinisciences

Product Information
Sequence	KNLNESLIDLQELGK
Purity	>90% (HPLC-MS)
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	Cellular immune response, T-cell expansion, Antigen specific T-cell stimulation, Immune monitoring, T-cell assays
Primary Accession	P0DTC2
Other Accession	AAP41037.1

Additional Information
Gene ID	4374056
Other Names	SARS-CoV-2 Spike Glycoprotein , E2 , Peplomer
Format	Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Protein Information
Name	S {ECO:0000255\|HAMAP-Rule:MF_04099}
Function	Spike glycoprotein is cleaved into S1, S2, and S3 chains. Spike protein S1 attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry . Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.Spike protein S2 mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Spike protein S2' acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
Cellular Location	Virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane

SARS-CoV-2 Spike Peptide (KNLNESLIDLQELGK)