SR Proteins (Non-snRNP Splicing Factor) (Biotin)

SR proteins are family of highly conserved arginine/serine rich, spliceosome associated phosphoproteins essential for metazoan pre-mRNA splicing (3,4). SR proteins appear to act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role, because they can determine alternative splice site usage in vivo and in vitro (5-7); a particularly interesting example being the regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins (8). SR proteins appear to be recruited from nuclear “speckles,” in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus (1,9). ||Applications: |Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.