Tau (Tau Protein, Microtubule-associated Protein, MAP) (BSA & Azide Free) (APC)

Referentie T1037-01X-APC-100ul

Formaat : 100ul

Merk : US Biological

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T1037-01X-APC Tau (Tau Protein, Microtubule-associated Protein, MAP) (BSA & Azide Free) (APC)

Clone Type
Polyclonal
Host
mouse
Source
bovine
Isotype
IgG1
Grade
Affinity Purified
Applications
IF IP WB
Crossreactivity
Bo Hu Mo Rt Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Tau proteins are microtubule-associated proteins (MAPs). Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. ||Applications:|Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Not suitable for use in Imunohistochemistry. Other applications have not been tested.||Recommended Dilutions: |Immunoprecipitation (Native verified): 2ug/mg protein lysate. Use Protein G.|Western Blot: 1-2ug/ml for 2 hours at RT |Optimal dilutions to be determined by the researcher.||Positive Control: |IMR5 cells, Human T98G glioblastoma cells or Alzheimer’s disease brain tissue ||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3F348|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from ascites|Immunogen: Purified bovine microtubule-associated proteins. MW of Antigen: 45-68kD. Epitope: Middle of Tau. Cellular Localization: Cytoplasmic.|Specificity: Recognizes bovine Tau. Recognizes both the nonphosphorylated and the phosphorylated forms of Tau. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Shows no crossreaction with other MAPs or tubulin. Species Crossreactivity: human, sheep, mouse and rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified bovine microtubule-associated proteins. MW of Antigen: 45-68kD. Epitope: Middle of Tau. Cellular Localization: Cytoplasmic.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from ascites
Specificity
Recognizes bovine Tau. Recognizes both the nonphosphorylated and the phosphorylated forms of Tau. Intensely stains the human neurofibrillary tangles, neuropil threads and neuritic plaques associated with Alzheimer’s disease. Shows no crossreaction with other MAPs or tubulin. Species Crossreactivity: human, sheep, mouse and rat
References
1. Lopresti P, et. al. 91995) Proc Natl Acad Sci USA, 92:10369–10373