Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay™ Kit

Referentie T3774-92-100T

Formaat : 100Tests

Merk : US Biological

Meer informatie aanvragen

Neem contact op met een lokale distributeur :


Telefoonnummer : +1 850 650 7790


T3774-92 Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay™ Kit

Clone Type
Polyclonal
Shipping Temp
RT
Storage Temp
-20°C

Oxidative attack of essential cell components by reactive oxygen species has been associated with several human diseases, such as atherosclerosis, cardiovascular diseases, diabetes, liver disorders, and inflammatory rheumatic diseases. Thiobarbituric Acid Reactive Substances (TBARS) are low-molecular-weight end products (mainly malondialdehyde, MDA) that are formed during the decomposition of lipid peroxidation products. Increased levels of TBARS have been demonstrated in these diseases. Simple, direct and accurate assays for TBARS find wide applications in research and drug discovery. TBARS assay is based on the reaction of TBARS with thiobarbituric acid (TBA) to form a pink colored product. The color intensity at 535nm or fluorescence intensity at (lex/em=560nm/585nm) is directly proportional to TBARS concentration in the sample.||Key Features:|Sensitive and accurate. Linear detection range|Colorimetric Assay: 1-30uM MDA|Fluorometric Assay: 0.1-1.5uM MDA||Applications:|Direct Assays: Serum, plasma, urine, saliva and other biological samples|Drug Discovery/Pharmacology: Effects of drugs on TBARS.||Kit Components:|T3774-92A: TBA Reagent, 1x25ml|T3774-92B: Trichloroacetic Acid, 10%, 1x25ml|T3774-92C: 6M MDA Standard, 1x1vial||Storage and Stability:|Store all reagents at -20°C. Avoid freeze/thaw cycles. Stable for 6 months after receipt.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Yagi, K. (1976). A simple fluorometric assay for lipoperoxide in blood plasma. Biochem. Res. 15: 212-216. 2. Satoh, K. (1978). Serum lipid peroxide in cerebrovascular disorder determined by a new colorimetric method. Clin. Chim. Acts 90: 37-43. 3. Okawa H. et al (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358.