Thyroxine ELISA Kit (Fish)
Referentie OKCA00349
Formaat : 96Wells
Merk : Aviva Systems Biology
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Telefoonnummer : +1 850 650 7790
| Predicted Species Reactivity | Fish | ||||||||||||||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | < 4 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Thyroxine ELISA Kit (Fish) (OKCA00349) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Fish Thyroxine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thyroxine and incubated. The Thyroxine found in the sample or standards competes with the biotinylated Thyroxine for limited binding sites on the immobilized anti-Fish Thyroxine antibody. Excess unbound biotinylated Thyroxine and sample or standard Thyroxine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thyroxine captured in the well and inversely proportional to the amount of Thyroxine which was contained in the sample or standard. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 20 ng/mL - 320 ng/mL | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 20 ng/ml | ||||||||||||||||||||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of fish T4. No significant cross-reactivity or interference between fish T4 and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | Tyroxine |
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| Alias Symbols | T4 |
| Protein Name | Thyroxine |