Total Cholesterol and Cholesteryl Ester Assay Kit is an assay kit for the detection and quantification of Total Cholesterol and Cholesteryl Ester concentrations in serum, plasma, animal tissue homogenates and cell lysates using a fluorescence microplate reader. Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Research Articles on Total Cholesterol and Cholesteryl Ester
| Target | Total Cholesterol and Cholesteryl Ester |
| Reactivity | General (All species) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 0.12 µmol/L - 30 µmol/L |
| Sensitivity | 0.12 µmol/L |
| Detection Method | Fluorometric |
| Assay Data | Quantitative |
| Sample Type | Serum, plasma, animal tissue homogenates and cell lysates. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |