TransLvTM Lentivirus qPCR Titration Kit
Referentie FV201-01
Formaat : 100rxns
Merk : TransGen Biotech
Neem contact op met een lokale distributeur :
Telefoonnummer : +1 850 650 7790
ManualTransLvTM Lentivirus qPCR Titration Kit provides a simple, rapid, sensitive and efficient method to determine the copy number of integrated proviral sequences in lentivirus transduced cells using qPCR method. Lentivirus titer can be calculated from integrated proviral copy number in the genome of the lentivirus transduction susceptible cells (such as HT1080, HEK-293T, etc.) by using the standard curve generated from qPCR.
• Simple operation and high sensitivity.
• Higher accuracy enabled by internal reference gene.
• Compatible with the second and third generation HIV-1 lentiviral packaging vectors.
• Good linear relationship in the range of 103-108 copies/μl.
Storage
at -20°C in the dark for one year.
Shipping
Dry ice (-70℃)
| Component | FV201-01 (100 rxns) |
| 2×TransLvTM Lentivirus qPCR Titration SuperMix | 1 ml |
| 10×GC enhancer | 200 μl |
| Provirus Gene Standards (P1-P6) (108-103 copies/μl) | 30 μl each |
| Reference Gene Standards (R1-R6) (108-103 copies/μl) | 30 μl each |
| Provirus Gene Primer Mix (5 μM) | 100 μl |
| Reference Gene Primer Mix (5 μM) | 100 μl |
| Passive Reference Dye（50×） | 40 μl |
| Nuclease-free Water | 1 ml |
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