TransNGS® Fragmentase DNA Library Prep Kit for Illumina®

Referentie KP231-01

Formaat : 12rxns

Merk : TransGen Biotech

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TransNGS® Fragmentase DNA Library Prep Kit for Illumina®

Catalog Number: KP231-01

Price:Please inquire first

Specification:
12 rxns
96 rxns

Product Details

The kit is designed to efficiently and quickly prepare DNA library from 1 ng to 1 μg double-stranded DNA for the  Illumina high-throughput sequencing platform. The kit completes the DNA fragmentation, end repair and A-tailing in  one step. The product can be directly used for adapter ligation without the purification steps. Due to the use of  fragmentation enzymes to fragment the genome, libraries of inserts of different sizes can be obtained by adjusting the  fragmentation time, which simplifies the operation process and shortens the library preparation time. And the kit is  suitable for library construction of DNA from different sources and inputs.

Features

• Applicable to a wide range of sample types.

• High library conversion rate. 

Applications

• Whole genome sequencing. 

• Target gene sequencing. 

• Exon sequencing / other targeted capture sequencing. 

• Metagenomic sequencing.

Storage

at -20°C for one year

Shipping

Dry ice (-70℃)

Product Composition

Component

    KP231-01(12 rxns) KP231-02(96 rxns)
Fragmentation Buffer(10×)60 μl480 μl
Fragmentation Enzyme Mix120 μl960 μl
Fragmentation Enhancer60 μl480 μl
TransNGS® Adapter for Illumina®(16μM)60 μl480 μl
Adapter Dilution Buffer600 μl5 ml
Adapter-ligation Buffer for Illumina240 μl4×480 μl
Adapter-ligation Enzyme60 μl480 μl
TransNGS® Library Amplification SuperMix(2×)300 μl4×600 μl
TransNGS® Universal Primer Mix for Illumina®60 μl480 μl
Library Elution Buffer2 ml4×4 ml
Nuclease-free Water1 ml
5 ml


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