Transporter 5® Transfection Reagent
Referentie 26008-50
Formaat : 50ml
Merk : Polysciences
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Telefoonnummer : +1 850 650 7790
DatasheetTransporter 5® Transfection Reagent
| Catalog Number | Unit Size | QTY | |
|---|---|---|---|
| 26008-1A | 1 mL | | |
| 26008-5 | 5 mL | | |
| 26008-50 | 50 mL | | |
Product Overview
Transporter 5® is a premium ready-to-use transfection reagent prepared from our popular linear polyethylenimine MAX (PEI MAX® 1 mg/mL). Transporter 5® effectively transfects mammalian and insect cells, especially HEK-293, CHO, and Sf9.
We understand every transfection is a major investment, so we designed Transporter 5 to be a reliable reagent in any process.
The quality of Transporter 5 begins with our production process. Transporter 5 combines our consistent PEI with the highest quality USP/NF reagents in sterile, single-use equipment. While standard methods use 0.2µm sterile-filters, we use 0.1µm to completely eliminate mycoplasma risk.
Our Quality Control ensures Transporter 5 is the most reliable reagent available. We developed our own scientifically-sound, quantitative performance assay for Transporter 5. Most commercial performance assays are based on qualitative assays like SEAP and GFP. These assays cannot ensure the high titers most scientists require. The few reagents that are tested quantitatively typically use undisclosed HDAC inhibitors to improve results. Transporter 5 is required to produce high titers without any supplementation.
Properties
References
Backliwal, G., Hildinger, M., Chenuet, S., Wulhfard, S., De Jesus, M., & Wurm, F. M. (2008). Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic acids research, 36(15), e96-e96.
Backliwal, G., Hildinger, M., Hasija, V., & Wurm, F. M. (2008). High‐density transfection with HEK‐293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnology and bioengineering, 99(3), 721-727.
Choosakoonkriang, S., Lobo, B. A., Koe, G. S., Koe, J. G., & Middaugh, C. R. (2003). Biophysical characterization of PEI/DNA complexes. Journal of pharmaceutical sciences, 92(8), 1710-1722.
Li, C., Akuta, T., Nakagawa, M., Sato, T., Shibata, T., Maruyama, T., ... & Arakawa, T. (2020). Agarose native gel electrophoresis for characterization of antibodies. International Journal of Biological Macromolecules, 151, 885-890.
Longo, P. A., Kavran, J. M., Kim, M. S., & Leahy, D. J. (2013). Transient mammalian cell transfection with polyethylenimine (PEI). In Methods in enzymology (Vol. 529, pp. 227-240). Academic Press.
Rajendra, Y., Kiseljak, D., Baldi, L., Wurm, F. M., & Hacker, D. L. (2015). Transcriptional and post‐transcriptional limitations of high‐yielding, PEI‐mediated transient transfection with CHO and HEK‐293E cells. Biotechnology progress, 31(2), 541-549.
Tan, S., Tao, Z., Loo, S., Su, L., Chen, X., & Ye, L. (2019). Non-viral vector based gene transfection with human induced pluripotent stem cells derived cardiomyocytes. Scientific reports, 9(1), 1-11.
Tom, R., Bisson, L., & Durocher, Y. (2008). Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteins. Cold Spring Harbor Protocols, 2008(3), pdb-prot4977.
Wulhfard, S., Baldi, L., Hacker, D. L., & Wurm, F. (2010). Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 148(2-3), 128-132.