Urea Nitrogen Detection Kit
Referentie OKAU00022-2PLATE
Formaat : 2plate
Merk : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Urea Nitrogen Detection Kit (OKAU00022) |
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| Application | ELISA-Sandwich | ||||||||||||||||||||||||||||||
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| ELISA Kit Linearity | Linearity was determined by taking two human serum samples with known urea nitrogen concentrations and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.
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| ELISA Kit Principle | The DetectX® Urea Nitrogen (also called BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. Please read the complete kit insert before performing this assay. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14. | ||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra Assay Precision Three human samples were further diluted in water and run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were:
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| ELISA Kit Component |
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| Additional Information | Background: Urea is a by-product of protein metabolism by the liver and is removed from the blood by the kidneys. Urea freely filters through the glomerulous but is reabsorbed by the renal tubules in a flow-dependent fashion. The higher the flow rate the greater amount of urea nitrogen is cleared from circulation and eliminated through the kidneys. As a result, the level of circulating urea nitrogen, along with serum creatinine, serves as a primary measure of kidney function. Normal adult Blood Urea Nitrogen (BUN) levels should be between 7 and 21 mg urea nitrogen per 100 mL blood (mg/dL). Azotemia, poor kidney function, will cause elevated BUN levels (>= 50 mg/dL) and is associated with acute kidney failure or injury, severe acute pancreatitis, congestive heart failure or gastrointestinal bleeding. Azotemia also can occur with dehydration, as a result of alcohol abuse, or high protein diets. Lower than expected BUN levels are usually not clinically predictive, but are primarily associated with liver disease or malnutrition, including malabsorption and low protein diets. Urine and saliva are considered to be acceptable non-invasive samples for measurement of urea nitrogen. Serum creatinine is another metabolic waste product freely filtered by the glumerulous, but does not undergo tubular reabsorption. Its steady rate of elimination is frequently used to generate an index or ratio with BUN values for normalized evaluations. Easy to use Serum Creatinine and Urinary Creatinine Detection Kits are also available. | ||||||||||||||||||||||||||||||
| :: | Detection Limit: 0.065 mg/dL | ||||||||||||||||||||||||||||||
| Reconstitution and Storage | RT | ||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Urine, Saliva and TCM | ||||||||||||||||||||||||||||||
| Sensitivity | 0.030 mg/dL |
| Gene Full Name | Urea Nitrogen |
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