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> Uridine triphosphate (trisodium salt) [19817-92-6]

Uridine triphosphate (trisodium salt) [19817-92-6]

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Uridine triphosphate (UTP) trisodium salt is a pyrimidine nucleoside triphosphate that is used as a substrate to synthesize RNA or as an energy source in metabolic reactions. Uridine triphosphate trisodium salt activates membrane-bound P2Y2 receptors.

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Uridine triphosphate trisodium salt Chemical Structure

Uridine triphosphate trisodium salt Chemical Structure

CAS No. : 19817-92-6

This product is a controlled substance and not for sale in your territory.

Based on 2 publication(s) in Google Scholar

Other Forms of Uridine triphosphate trisodium salt:

  • Uridine triphosphate Obtenir un devis
Description

Uridine triphosphate (UTP) trisodium salt is a pyrimidine nucleoside triphosphate that is used as a substrate to synthesize RNA or as an energy source in metabolic reactions. Uridine triphosphate trisodium salt activates membrane-bound P2Y2 receptors[1][2].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Uridine triphosphate (UTP) (0, 10, 100, 250, 500, and 1000 µM; 2, 4, 6, 8, 10, and 12 h) trisodium salt enhances RT4-D6P2T cells migration and wound repair. Uridine triphosphate trisodium salt induces Schwannoma cell migration through activation of P2Y2 receptors and through the increase of extracellular MMP-2 activation and expression[2].
Uridine triphosphate (UTP) trisodium salt evokes MAPK phosphorylation in a biphasic pattern MAPK phosphorylation, with an early transient phosphorylation 5 min after treatment, and a late and sustained phosphorylation that appeared at 6 h and lasted up to 24 h[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Uridine triphosphate trisodium salt Related Antibodies

Western Blot Analysis[2]

Cell Line: RT4-D6P2T cells
Concentration: 250 µM
Incubation Time: 5 min, 45 min, 2 h, 6 h, 12 h, 24 h, 48 h
Result: Induces biphasic MAPK phosphorylation.
In Vivo

Uridine triphosphate (UTP) (0.44-4.4 µg/kg; i.v.; once) trisodium salt reduces infarct size and improves rat heart function after myocardial infarct[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats (250-300 g; 2-3 months) with myocardial infarction (MI)[3]
Dosage: 0.44 µg/kg 30 min before MI, and 4.4 µg/kg i.v. 24 h prior to MI.
Administration: i.v.; once
Result: Reduced infarct size and improves rat heart function after myocardial infarct.
Essai clinique
Masse moléculaire

550.09

Formule

C9H12N2Na3O15P3
CAS No.

19817-92-6
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O)[C@H](N2C(NC(C=C2)=O)=O)O[C@@H]1COP(OP(OP([O-])([O-])=O)([O-])=O)(O)=O.[3Na+]
Structure Classification
  • Ketones, Aldehydes, Acids
Initial Source
  • Endogenous metabolite
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvant et solubilité
In Vitro: 

H2O : 75 mg/mL (136.34 mM; Need ultrasonic)

DMSO : 10 mg/mL (18.18 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8179 mL 9.0894 mL 18.1788 mL
5 mM 0.3636 mL 1.8179 mL 3.6358 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1 mg/mL (1.82 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 1 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1 mg/mL (1.82 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 1 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (181.79 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation

Purity: ≥97.0%

COA (254 KB) HNMR (117 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. Choi JH, et al. Uridine triphosphate increases proliferation of human cancerous pancreatic duct epithelial cells by activating P2Y2 receptor. Pancreas. 2013 May;42(4):680-6.  [Content Brief]

    [2]. Lamarca A, et al. Uridine 5'-triphosphate promotes in vitro Schwannoma cell migration through matrix metalloproteinase-2 activation. PLoS One. 2014 Jun 6;9(6):e98998.  [Content Brief]

    [3]. Yitzhaki S, et al. Uridine-5'-triphosphate (UTP) reduces infarct size and improves rat heart function aftermyocardial infarct. Biochem Pharmacol. 2006 Oct 16;72(8):949-55.  [Content Brief]

  • [1]. Choi JH, et al. Uridine triphosphate increases proliferation of human cancerous pancreatic duct epithelial cells by activating P2Y2 receptor. Pancreas. 2013 May;42(4):680-6.

    [2]. Lamarca A, et al. Uridine 5'-triphosphate promotes in vitro Schwannoma cell migration through matrix metalloproteinase-2 activation. PLoS One. 2014 Jun 6;9(6):e98998.

    [3]. Yitzhaki S, et al. Uridine-5'-triphosphate (UTP) reduces infarct size and improves rat heart function aftermyocardial infarct. Biochem Pharmacol. 2006 Oct 16;72(8):949-55.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.8179 mL 9.0894 mL 18.1788 mL 45.4471 mL
5 mM 0.3636 mL 1.8179 mL 3.6358 mL 9.0894 mL
10 mM 0.1818 mL 0.9089 mL 1.8179 mL 4.5447 mL
15 mM 0.1212 mL 0.6060 mL 1.2119 mL 3.0298 mL
H2O 20 mM 0.0909 mL 0.4545 mL 0.9089 mL 2.2724 mL
25 mM 0.0727 mL 0.3636 mL 0.7272 mL 1.8179 mL
30 mM 0.0606 mL 0.3030 mL 0.6060 mL 1.5149 mL
40 mM 0.0454 mL 0.2272 mL 0.4545 mL 1.1362 mL
50 mM 0.0364 mL 0.1818 mL 0.3636 mL 0.9089 mL
60 mM 0.0303 mL 0.1515 mL 0.3030 mL 0.7575 mL
80 mM 0.0227 mL 0.1136 mL 0.2272 mL 0.5681 mL
100 mM 0.0182 mL 0.0909 mL 0.1818 mL 0.4545 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Uridine triphosphate trisodium salt Related Classifications

  • Oligonucleotides
  • Nucleotides and their Analogs
  • Metabolic Enzyme/Protease GPCR/G Protein
  • Endogenous Metabolite P2Y Receptor
Help & FAQs

Keywords:

Uridine triphosphate trisodium19817-92-6UTP trisodium Uridine 5'-triphosphate trisodiumEndogenous MetaboliteP2Y ReceptorInhibitorinhibitorinhibit

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