Specifications

Product Data
Clone Name	B608M
Applications	ELISA, IF
Recommended Dilution	ELISA. IFA.
Reactivities	Vaccinia virus
Host	Mouse
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Native, intact Lister strain of Vaccinia virus
Specificity	This antibody recognizes free Vaccinia virus and infected cells. Reactive with Lister and NYCBH strains.
Formulation	0.01M PBS, pH 7.2 containing 0,09% sodium azide State: Purified State: Liquid purified Ig
Concentration	lot specific
Purification	>90% pure. Protein A chromatography
Conjugation	Unconjugated
Storage	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Background	Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs).
Reference Data