Vash1 (NM_177354) Mouse Tagged ORF Clone
Referentie MR222520
Formaat : 10ug
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| Product Data | |
| Target Symbol | Vash1 |
|---|---|
| Synonyms | AI834978; D930046M13Rik; G630009D10Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >MR222520 representing NM_177354 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGGGGGAAAGAAGGTGGTCCCAAGTGGCAGCAGCAGTGCCTCCCCGAACGCAGCCGCCACCACCA CTGCTGCTGCTGCTGCTGCCGCTGCTGCCCCCCACTCTGGCACTAAACGTTTGGAGACCACCGAAGGGGC CTCAGCACAGAGAGATGAGGAACCCGAAGAGGAAGGGGAAGAGGACCTACGAGATGGAGGTGTCCCTTTT TTCATCAACCGAGGTGGACTGCCAGTGGATGAGGCCACCTGGGAAAGGATGTGGAAGCATGTGGCCAAGA TCCACCCAGATGGAGAGAAGGTGGCCCTGCGCATCCGTGGGGCCACCGACCTGCCCAAGATTCCCATACC AAGTGTGCCTACTTTCCAGCCGACGACGCCCGTCCCTGAGCGCCTGGAAGCCGTGCAGCGCTACATCAGG GAGCTGCAGTACAATCACACAGGGACACAATTCTTTGAAATTAAGAAGAGCAGACCTCTGACGGGGCTGA TGGACCTGGCCAAGGAAATGACCAAAGAGGCTCTGCCAATCAAATGCCTGGAAGCTGTGATCCTGGGAAT TTACCTCACCAACAGTATGCCCACCCTGGAACGCTTTCCCATCAGCTTCAAGACCTATTTCTCAGGGAAC TACTTCCGCCACATTGTGCTGGGGGTGAACTTCGGGGGCCGCTATGGCGCCCTGGGTATGAGCCGAAGGG AGGACCTGATGTACAAACCGCCCGCCTTCCGCACGCTCAGTGAGCTCGTGCTGGACTACGAAGCGGCCTA CGGCCGCTGCTGGCACGTGCTGAAGAAGGTGAAGCTGGGTCAGTGTGTGTCCCACGACCCGCACAGTGTG GAACAGATTGAATGGAAGCACTCGGTGCTGGATGTGGAGAGGCTGGGGCGGGAAGACTTCCGCAAGGAGC TGGAGCGTCATGCCCGAGACATGCGGCTCAAGATTGGCAAGGGGACAGGCCCTCCTTCTCCCACCAAGGA CCGGAAGAAGGATGTTTCCTCCCCACAGAGGGCCCAGTCCAGCCCCCATCGCAGGAACAGCCGCAGTGAG AGACGGCCTTCAGGGGAGAAGAAGCCCGCAGAGCCCAAAGCCATGCCAGACCTCAGTGGGTACCAGATCC GGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >MR222520 representing NM_177354 Red=Cloning site Green=Tags(s) MPGGKKVVPSGSSSASPNAAATTTAAAAAAAAAPHSGTKRLETTEGASAQRDEEPEEEGEEDLRDGGVPF FINRGGLPVDEATWERMWKHVAKIHPDGEKVALRIRGATDLPKIPIPSVPTFQPTTPVPERLEAVQRYIR ELQYNHTGTQFFEIKKSRPLTGLMDLAKEMTKEALPIKCLEAVILGIYLTNSMPTLERFPISFKTYFSGN YFRHIVLGVNFGGRYGALGMSRREDLMYKPPAFRTLSELVLDYEAAYGRCWHVLKKVKLGQCVSHDPHSV EQIEWKHSVLDVERLGREDFRKELERHARDMRLKIGKGTGPPSPTKDRKKDVSSPQRAQSSPHRRNSRSE RRPSGEKKPAEPKAMPDLSGYQIRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_177354 |
| ORF Size | 1125 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177354.4, NP_796328.2 |
| RefSeq Size | 6189 bp |
| RefSeq ORF | 1128 bp |
| Locus ID | 238328 |
| UniProt ID | Q8C1W1 |
| Cytogenetics | 12 D2 |
| MW | 42.3 kDa |
| Summary | Tyrosine carboxypeptidase that removes the C-terminal tyrosine residue of alpha-tubulin, thereby regulating microtubule dynamics and function (PubMed:29146868). Acts as an angiogenesis inhibitor: inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis (PubMed:19204325). This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts (By similarity).[UniProtKB/Swiss-Prot Function] |
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