Vegfc Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommended Dilution	ELISA: 2.5 µg/ml, allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C.  Western Blot: 2-5 μg/ml. Immunoprecipitation: 1-10 µg / ml lysate or reaction volume.
Reactivities	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Rat VEGF-C native protein.(Asp101-Ile221) derived from insect cells (Cat.-No DA3519X)
Specificity	The antibody will react with Rat VEGF-C and will cross-react with Human and Mouse VEGF-C proteins.
Formulation	PBS, pH 7.4, containing no preservative. State: Azide Free State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of > 0.5 mg/ml.
Purification	Protein A affinity chromatography.
Conjugation	Unconjugated
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	vascular endothelial growth factor C
Database Link	Entrez Gene 114111 Rat UniProt ID O35757
Background	VEGF-C, a member of the VEGF/PDGF family of structurally related proteins, is a potent angiogenic cytokine. It promotes endothelial cell growth, promotes lymphangiogesis, and can also affect vascular permeability. VEGF-C is expressed in various tissues, but is not produced in peripheral blood lymphocytes. It forms cell surfaced-associated non-covalent disulfide linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. During embryogenesis, VEGF-C may play a role in the formation of the venous and lymphatic vascular systems. Both VEGF-C and VEGF-D are over-expressed in certain cancers, and the resulting elevated levels of VEGF-C or VEGF-D tend to correlate with increased lymphatic metastasis. Recombinant, fully processed rat VEGF-C is a 16-18 kDa non-disulfide linked homodimeric protein consisting of two 116 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.
Synonyms	VEGFC, VRP, Vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, Flt4 ligand
Reference Data