Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity)

Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity), a luminol-based chemiluminescent substrate is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP)-conjugated secondary antibodies. It is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates.

Target	Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity)
Tested Applications	WB
Storage	Store at room temperature for up to 24 months. Avoid exposure to light.
Buffer	Not applicable.
Availability	Shipped within 10-15 working days.
Note	This product is for research use only.
Directions for use	Keep the membrane moist in the wash buffer while preparing the substrate mixture. Please ensure the membrane does not dry out during the subsequent steps. Mix Luminol solution and Peroxide Solution in a 1:1 ratio, and thoroughly mix the chemiluminescent substrate solution well. Prepare 0.1 ml of solution per cm2 of membrane. For a mini-sized membrane (7 × 8.5 cm), 5 ml of solution is sufficient. For a midi-sized membrane (8.5 × 13.5 cm), 10 ml of solution is sufficient. Place the membrane with the protein side up on a clear and level surface or in a clean container. Remove the membrane from the chemiluminescent substrate solution and drain off excessive solution. Place the membrane in a plastic sheet protector or in plastic wrap to prevent the membrane from drying. Image the membrane with a digital imager or by exposure to X-ray film.

Research Articles on Western Enhanced Chemiluminescent (ECL) Substrate (Low Femto Sensitivity)