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F(ab) fragmented secondary antibodies
Fab and F(ab')2 fragments are two types of fragmented secondary antibodies that are commonly used in various applications. Here are some key points about F(ab) fragments:
- F(ab) fragments are monovalent antibody fragments that contain only one antigen-binding Fab portion and lack the Fc region. They are generated by papain digestion of whole IgG antibodies to remove the Fc region and the entire hinge region.
- F(ab) fragments have a molecular weight of about 50 kDa, which is smaller than whole IgG antibodies and F(ab')2 fragments. This allows for better penetration into tissues and reduces nonspecific binding to Fc receptors on live cells or to Protein A/G.
- F(ab) fragments are useful for blocking endogenous immunoglobulins on cells, tissues, or other surfaces, and for blocking the exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. They can also be used to Fab-label primary antibodies prior to incubation with the experimental sample.
- F(ab) fragments are monovalent and have lower avidity than F(ab')2 fragments, which may affect their sensitivity and specificity in certain applications.
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- Unconjugated 185
- FITC 49
- HRP 39
- Biotin 37
- TRITC 22
- AMCA 14
- Cy3 14
- Cy5 14
- Cy5.5 14
- Cy7 14
- Red 14
- AP 12
- Alexa Fluor 350 10
- Alexa Fluor 405 10
- Alexa Fluor 532 10
- Alexa Fluor 555 10
- Alexa Fluor 568 10
- Alexa Fluor 633 10
- Alexa Fluor 647 10
- Alexa Fluor 660 10
- Alexa Fluor 680 10
- Alexa Fluor 750 10
- PE 7
- APC 6
- AbFluor™ 488 6
- AbFluor™ 594 6
- Alexa Fluor 790 6
- Alexa Fluor 488 4
- Alexa Fluor 594 4
- Allophycocyanin 1
- PerCp 1
- R-Phycoerythrin 1
- human 334
- mouse 27
- rat 27
- rabbit 26
- goat 21
- chicken 20
- hamster 20
- sheep 20
- guinea pig 19
- Secondary antibody
- Secondary Antibody 4
- goat 235
- mouse 165
- rabbit 89
- donkey 30
- human 28
- monkey 6
- rat 6
- guinea pig 3
- chicken 1
- IHC 436
- IF 352
- FC 328
- ELISA 215
- WB 185
- FLISA 73
- Microscopy 53
- ICC 30
- IP 26
- Dot blot 16
- Polyclonal 269
- Monoclonal 98
- polyclonal 76
- NI 6014 (HP 6014) 6
- MA2 4
- 4A11 2
- NI 330 (HP 6050) 2
- RMG01 2
- RMG05 2
- 1105CT1.1.1 1
- 7HA13 1
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