HRP Chromogen for IHC - AEC
AEC (Aminoethyl carbazole) is one of the most used chromogens for immunohistochemistry (IHC) using horseradish peroxidase (HRP). HRP catalyzes the formation of a red precipitate from AEC.
The AEC is easy to use, simply incubate the slides for 5-10 minutes in a humid chamber at room temperature. The appearance of the staining can be followed by observation under microscope.
Since AEC is soluble in alcohol, samples should not be dehydrated after staining.
Slides can be mounted using an aqueous mounting medium (Simpo-Mount, NeoMount ...).
AEC can be used as a contrast stain with tissue pigments or for multiple labeling with other chromogens.
Name
AEC (Aminoethyl carbazole)
Applications
IHC, ICC, ISH, WB, ELISA
Detection enzyme
HRP
Reaction type
Precipitating Reaction
Precipitate color
Red
Microscopy
Brightfield, Spectral Imaging
Mounting
Aqueous
Other properties
Contrast with Tissue Pigments, Multiple Labeling
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- Unconjugated 2126
- HRP 7
- APC 6
- Biotin 6
- FITC 6
- MaxLight 490 6
- MaxLight 550 6
- MaxLight 650 6
- PE 6
- MaxLight 405 4
- MaxLight 750 4
- human 81
- cow 67
- bacteria 63
- pig 40
- primary 15
- mouse 14
- rat 12
- e. coli 11
- yeast 6
- chicken 4
- dog 4
- guinea pig 4
- monosan aec substrate 2
- 1 1
- monosan permanent aec 1
- monosan plus (hrp) broad spectrum (aec)kit 1
- yeast 1443
- 850
- e. coli 254
- mouse 62
- bacteria 40
- rabbit 40
- rat 9
- human 8
- dog 2
- goat 1
- virus 1
- Protein/peptide 5990
- Biochemicals 215
- Primary antibody 76
- ELISA/ELISPOT 42
- Inhibitor/Antagonist/Agonist 39
- Cell line 20
- Buffers and reagents 18
- Primary cells 15
- kit 8
- pcr products 6
- Genomic DNA 2
- ELISA 69
- WB 65
- IHC 55
- IF 40
- FLISA 16
- PCR 8
- ICC 4
- FC 3
- ELISPOT 2
- Chromatography 1
- IP 1
- ISH 1
- 5C4 32
- 2F7 30
- Not Applicable 2
- Polyclonal 2
- AAEC-19 1
- AEC-16 1
- AECB-3 1
- AECC-14 1
- AECE-1 1
- AECF-19 1
- AECG-9 1
- AECH-22 1
- AECI-19 1
- AECO-3 1
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