Product Description  

Vazyme LAmp DNA Polymerase is a mixed enzyme of Taq DNA Polymerase and a protein containing 3'→5' exonuclease activity (proofreading activity). The fidelity is 6-fold higher than Taq DNA Polymerase. With a specially optimized buffer system, Vazyme LAmp DNA Polymerase is suitable for Long-range PCR (up to 21 kb when using the genome as a template). It has high amplification efficiency for templates of different origins and lengths. 2 × Vazyme LAmp Master Mix contains Vazyme LAmp DNA Polymerase, dNTP and optimized buffer system. It only needs to add primers and templates to perform amplification reaction, thereby reducing pipetting operations and improving detection throughput and reproducibility of results. The system contains protective agents that keep 2 × Master Mix stable in activity after repeated freezing and thawing. This product is available in a version containing loading buffer and tracking dye, so PCR products can be directly loaded for electrophoresis after the reaction, which is convenient to use. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/ C116/C603).

 Features  

• Good performance: ~6X higher than Taq DNA Polymerase; higher amplification efficiency

• Convenient operation: Ready-to-use mix; include tracking dye, direct loading of PCR products on a gel

• Additional features: 3′→5′ exonuclease activity

• Broad Compatibility: Amplify fragments up to 21 kb (gDNA)

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.