ACP5 ELISA Kit (Mouse)
Referentie OKEH03244
Formaat : 96Wells
Merk : Aviva Systems Biology
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| Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ACP5 ELISA Kit (Mouse) (OKEH03244) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Acp5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Acp5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Acp5 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20U/L | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 7.1% (n = 20) Mean Inter-assay CV%: < 10.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 0.156U/L | ||||||||||||||||||||||
| Specificity | Natural and recombinant Mouse Tartrate-resistant acid phosphatase type 5 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Acp5 |
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| Gene Full Name | acid phosphatase 5, tartrate resistant |
| Alias Symbols | acid phosphatase type V;T;tartrate-resistant acid ATPase;tartrate-resistant acid phosphatase type 5;TR;TRACP;TRAP;TR-AP;trATPase;type 5 acid phosphatase. |
| NCBI Gene Id | 11433 |
| Protein Name | Q05117 |
| Description of Target | Tartrate-resistant acid phosphatase type 5 |
| Uniprot ID | https://www.uniprot.org/uniprot/Q05117 |
| Protein Accession # | NP_001095874.1 |
| Nucleotide Accession # | NM_001102404.1 |
| Protein Size (# AA) | 327 |
| Molecular Weight | 37 kDa |