General Info
| Host: | Mouse |
| Applications: | FC/IHC-F/IF |
| Reactivity: | Human/Mouse/Pig/Sheep/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-CD28 is suitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: | Monoclonal |
| Clone ID: | 204.12 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b |
| Formulation: | PBS with 0.02% sodium azide |
| Concentration: | 100 ug/mL |
| Dilution Range: | FC 0 5-1 0 µg/million cells in 0 1 ml-IF 0 5-1 0 µg/ml-IHC frozen 1-2 µg/ml for 30-60 min at RT |
| Storage Instruction: | Store at 2-8°C upon receipt. |
Information
| Gene Symbol: | CD28 |
| Gene ID: | 940 |
| Uniprot ID: | CD28_HUMAN |
| Immunogen: | Stimulated human leucocytes |
Description
| Tissue Specificity | Expressed in T-cells and plasma cells, but not in less mature B-cells. |
| Post Translational Modifications | CD40LG induces tyrosine phosphorylation of isoform 3. |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation. Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. |
| Protein Name | T-Cell-Specific Surface Glycoprotein Cd28Tp44Cd Antigen Cd28 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-164939Reactome: R-HSA-2219530Reactome: R-HSA-389356Reactome: R-HSA-389357Reactome: R-HSA-389359Reactome: R-HSA-6811558 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinIsoform 3: Cell Surface |
| Alternative Antibody Names | Anti-T-Cell-Specific Surface Glycoprotein Cd28 antibodyAnti-Tp44 antibodyAnti-Cd Antigen Cd28 antibodyAnti-CD28 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance