A5001-300 | ApexPrep DNA Plasmid Miniprep Kit Clinisciences

Background

The ApexPrep DNA Plasmid Miniprep kit is based on alkaline lysis technology, which can lyse cells and then adsorb DNA to the adsorption membrane in the presence of high salt. Subsequent steps such as washing away impurities can efficiently and quickly extract up to 30 μg of plasmid DNA. This kit uses a unique buffer formula to maximize the removal of protein impurities and other organic compounds, and can process 1-5 mL of bacterial solution each time. The yield and quality of plasmid extraction are related to the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics, and other factors.

Plasmid DNA prepared by ApexPrep DNA Plasmid Miniprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and trans-formation, in vitro translation, and transfection of robust cells.

Product Citation

1. Xiang Song Jian Gong, et al. "Plasma-based early screening and monitoring of EGFR mutations in NSCLC patients by a 3-color digital PCR assay." Br J Cancer. 2020 Oct;123(9):1437-1444. PMID:32782293
2. Wang EX, Ding MZ, et al. "Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation." Microb Cell Fact. 2016 Jan 25;15:21. PMID:26809519

Chemical Properties

Storage	Store at room temperature for one year
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Components and Storage

Components	50 tests	300 tests
Buffer BL	30 mL	160 mL
Buffer A1	20 mL	100 mL
Buffer A2	20 mL	100 mL
Buffer A3	30 mL	120 mL
Buffer AP	12 mL	50 mL
Buffer AE	15 mL	30 mL
RNase A	200 μL (10mg/mL)	1 mL (10mg/mL)
Spin Columns	50	300
Collection Tubes	50	300
Store at room temperature for one year. Buffer A1 with RNase A should be stored at 2-8°C.

Description

For purification of up to 20 μg molecular biology grade plasmid DNA

Ready-to-use plasmid DNA in minutes
Reproducible yields of molecular biology grade plasmid DNA
Single protocol for high- and low-copy vectors

The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.

ApexPrep DNA Plasmid Miniprep Kit

Referentie A5001-300

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