Images
Flow Cytometry
Application

Flow Cytometry

Chicken peripheral blood mononuclear cells were double stained with mouse anti-chicken CD3-R-PE and Bu-1 monoclonal antibody, clone AV20 (FITC) (Cat # MAB5853). Lymphocytes were then gated and the cells analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against native Bu-1.

    Immunogen

    Native purified from Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens.

    Host

    Mouse

    Reactivity

    Chicken

    Specificity

    Chicken Bu-1 (chB6), Mr 70-75 KDa.

    Form

    Liquid

    Conjugation

    FITC

    Isotype

    IgG1, kappa

    Recommend Usage

    Flow Cytometry (1 ug/106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Frozen sections)

    Immunoprecipitation

    Flow Cytometry

    Chicken peripheral blood mononuclear cells were double stained with mouse anti-chicken CD3-R-PE and Bu-1 monoclonal antibody, clone AV20 (FITC) (Cat # MAB5853). Lymphocytes were then gated and the cells analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
  • Publication Reference