Flow cytometry: 1/25-1/200; Use 10 µl to label 5x10e5 cells. Immunohistochemistry on Paraffin sections: This product does not require protein digestion pretreatment nor antigen retrieval using heat treatment prior to staining of paraffin sections. Functional assays. Does not work on frozen sections or in immunoprecipitation.
Reactivity
Bovine, Human, Rabbit
Antibody Host
Mouse
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Synthetic peptide comprising the N-terminal extracellular domain (Met1 - Asn31) of the C5a receptor (C5aR) CD88. Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Specificity
The antibody recognises the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 (raised against a synthetic peptide Met1 -Asn31) has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 inhibits C5a binding to its receptor.
Buffer
PBS without stabilizer or preservative State: Azide Free State: Liquid purified IgG
Concentration
lot specific
Purification
Protein G chromatography
Conjugation
Unconjugated
Storage
Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
The human anaphylatoxin C5a is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
