Caspase 8 (CASP8) (176-460) Mouse Monoclonal Antibody [Clone ID: 5F7]
CAT#: AM26588AF-N
Caspase 8 (CASP8) (176-460) mouse monoclonal antibody, clone 5F7, Azide Free
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Specifications
Product Data | |
Clone Name | 5F7 |
Applications | WB |
Recommended Dilution | Western blot: 1:1000 for chemiluminescence detection system. For details see protocol below. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | GST-FLICE fusion protein corresponding to C-terminal amino acids of human FLICE (176-460 a.a.) |
Specificity | This antibody reacts with caspase 8 on Western blotting. It doesn't cross-react with other caspases including caspase10 (FLICE2) which has high homology to caspase 8, however cross-reactivity with I-FLICE has not been examined. The antibody detects 55 KD of human caspase 8a (MACH α 1) as well as 54KD of human caspase 8b (MACH α 2) on Western blotting with total cell lysate from Jurkat, Raji, U937 and HeLa. Also detects the 18 kDa active form, and the 43 and 26 kDa intermediate forms. May detect a n unidentiffied 72 kDa band in some cell lines. |
Formulation | Protein-A Sepharose, PBS containing 50% glycerol. Contains no preservatives. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | caspase 8 |
Database Link | |
Background | Caspase 8 (FLICE/MACH/Mch5) is a member of the ICE (interleukin-1 β converting enzyme)/CED-3 family cysteine protease. It is the most upstream protease that receives the activation signal from the Fas (APO1/CD95) and TNFR1 (Tumor Necrosis Factor Receptor 1) to initiate the apoptotic protease cascade that leads to activation of ICE/CED-3 family proteases. Caspase 8 has high homologous reagion to the ICE/CED-3 family in C-terminal and two death effecter domains (DED) in N-terminal. Binding of caspase 8 to FADD (MORT1) through association of their DED, and consequent activation of the caspases by their proteolytic cleavage, are thought to be critical steps in the initiation of Fas- and TNFR1-induced apoptosis 1) 2) 3) . Recently the inhibitor of Fas- and TNFR1-induced apoptosis is identified, called I- FLICE (FLIP/Casper/ FLAME/CASH). I-FLICE has high homology to caspase 8 and it contains two DED, which interacts with caspase 8 4) and FADD 5) , and potently inhibits Fas- and TNFR1-induced apoptosis. |
Synonyms | CASP-8, CASP8, MCH5, CAP4 |
Note | This product was originally produced by MBL International. Protocol: SDS PAGE & Western Blotting |
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