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> CD45R antibody [RA3-6B2] (PE)

CD45R antibody [RA3-6B2] (PE)

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Referentie GTX00486-08-100ug

Formaat : 100ug

Merk : GeneTex

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Anti-CD45R antibody [RA3-6B2] (PE) used in Flow cytometry (FACS). GTX00486-08

Host

 Rat

Clonality

 Monoclonal

Clone Name

 RA3-6B2

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human, Mouse, Cat
Package
100 μg
Datasheet File
View product citations for antibody GTX00486-08 on CiteAb
PRODUCT

Summary

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Product Note

This antibody recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin (PE) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase, receptor type, C , B220 , CD45R , Cd45 , L-CA , Ly-5 , Lyt-4 , T200 , loc

Cellular Localization

Cell membrane

Database

DATA IMAGES

Anti-CD45R antibody [RA3-6B2] (PE) used in Flow cytometry (FACS). GTX00486-08

GTX00486-08 FACS Image

FACS analysis of C57Bl/6 splenocytes using GTX00486-08 CD45R antibody [RA3-6B2] (PE).
Solid line : Primary antibody
Dashed line : PE rat IgG2a isotype control
Antibody amount : 0.5 μg

Clone Reference

2012; A role for GPx3 in activity of normal and leukemia stem cells
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2011; Loss of Roquin induces early death and immune deregulation but not autoimmunity
2010; Widespread overexpression of epitope-tagged Mdm4 does not accelerate tumor formation in vivo
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
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REVIEW

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Clone Reference
2012; A role for GPx3 in activity of normal and leukemia stem cells
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2011; Loss of Roquin induces early death and immune deregulation but not autoimmunity
2010; Widespread overexpression of epitope-tagged Mdm4 does not accelerate tumor formation in vivo
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
2004; Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes
1996; Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues
1995; Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies
SDS
PBS.pdf
Sodium Azide.pdf