CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7) (PE/CY5)

Referentie C2432-14E-100ug

Formaat : 100ug

Merk : US Biological

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C2432-14E CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7) (PE/CY5)

Clone Type
Polyclonal
Host
rat
Source
mouse
Isotype
IgG2a,k
Grade
Purified
Applications
FC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD80 (B7-1) is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed by dendritic cells, monocytes, and peritoneal macrophages. Expression of CD80 on B cells is upregulated after activation by various means including stimulation by LPS, IL-4, and surface Ig receptor cross linking with peak expression occurring between 48 and 72 hours postactivation. 1-4 The interaction of CD80/B7-1 with its ligands, CD28 and CD152/CTLA-4, provides a potent costimulatory signal for T cells activated through the CD3/T cell receptor complex.5, 6 This antibody locks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigen presenting cells. 2, 7||Applications:|• Flow cytometry 2, 7|• Immunoprecipitation 7|• In vivo and in vitro blocking of ligand binding 7||Recommended Dilution:|Flow Cytometry: ≤ 0.2ug/10e6 cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 1G10|Host: rat|Source: mouse|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.|Purity: Purified|Specificity: Recognizes mouse CD80 (B7-1), Mr 45-47kD.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
Purity
Purified
Specificity
Recognizes mouse CD80 (B7-1), Mr 45-47kD.
References
1. Nabavi, N., G. J. Freeman, A. Gault, D. Godfrey, L. M. Nadler, and L. H. Glimcher. 1992. Nature 360:266|2. Chen, C., D. A. Faherty, A. Gault, S. E. Connaughton, G. D. Powers, D. I. Godfrey, and N. Nabavi. 1996. J. Immunol. 152:2105.|3. Natesan, M., Z. Razi-Wolf, and H. Reiser. 1996. J. Immunol. 156:2783.|4. Razi-Wolf, Z., G. J. Freeman, F. Galvin, B. Benacerraf, L. Nadler, and H. Reiser. 1992. Proc. Natl. Acad. Sci. U. S. A. 89:4210.|5. Hathcock, K. S., G. Laszlo, C. Pucillo, P. Linsley, and R. J. Hodes. 1994. J. Exp. Med. 180:631.|6. Bluestone, J.A. 1995. Immunity 2:255.|7. Powers, G. D., D. A. Faherty, S. E. Connaughton, D. A. Biondi, D. I. Godfrey, A. Gault, C. Y. Chen, and N. Nabavi. 1994. Cell Immunol. 153:298.