Factor XIIIa (Fibrinoligase, Plasma Transglutaminase, Fibrin Stabilizing Factor) (BSA & Azide Free) (HRP)

Factor XIII is a beta-globulin found in plasma. It is composed of two subunits. Factor XIIIa is the catalytic subunit. It is a dimer of MW 160kD. Factor XIII is present in plasma as an a2b2 heterodimer (MW 320kD). In platelets, only the a2 unit exists. Factor XIIIa is a dermal dendrocyte marker. It shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system. Factor XIII has also been used with CD34 to differentiate between dermatofibroma and dermatofibrosarcoma protuberans.||Applications:|Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilution: |Western Blot: 1-2ug/ml for 2hrs at RT. |Immunohistochemistry (Formalin/paraffin): 1:100 for 20 minutes at RT using the LP system, 30 minutes at RT using UltraVision. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.|Optimal dilutions to be determined by the researcher.||Positive Control: |Recombinant human Factor XIII a-subunit, capillary hemangioma, dermatofibroma and placenta. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.