GENIUS™ Nuclease [9025-65-4]
Referentie sc-202391
Formaat : 25KU
Merk : Santa Cruz Biotechnology
GENIUS™ Nuclease (CAS 9025-65-4)
Application:
Numéro CAS:
9025-65-4
Pureté:
≥90%
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
ACCÈS RAPIDE AUX LIENS
Informations pour la commande
Références bibliographiques
Description
Information Technique
Données de Sécurité
SDS & Certificat d’Analyses
GENIUS™ Nuclease is an engineered enzyme tailored for genome editing applications in molecular biology and genetic research. This tool is specifically designed to enhance the precision and efficiency of DNA manipulation by targeting and cleaving specific sequences within the genome. Its mechanism involves the recognition of designated DNA regions followed by the introduction of double-stranded breaks, facilitating targeted genetic modifications such as gene knockouts, insertions, and tagging. The use of GENIUS™ Nuclease in research allows scientists to precisely edit the genetic material, enabling the exploration of gene functions, the study of genetic disorders, and the development of genetically modified organisms. This nuclease′s ability to execute precise genetic alterations significantly contributes to advancements in genetic engineering by improving the accuracy, reliability, and speed of experimental outcomes. It stands as a critical asset in the field of genetic research, providing researchers with the capability to perform complex genetic rearr
References:
- Disulfide bonds are required for Serratia marcescens nuclease activity. | Ball, TK., et al. 1992. Nucleic Acids Res. 20: 4971-4. PMID: 1329033
- The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. | Ball, TK., et al. 1987. Gene. 57: 183-92. PMID: 3319779
- 2.1 A structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA. | Miller, MD., et al. 1994. Nat Struct Biol. 1: 461-8. PMID: 7664065
- A new mass spectrometric approach to detect modifications in DNA. | Janning, P., et al. 1994. Rapid Commun Mass Spectrom. 8: 1035-40. PMID: 7696699
- Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis. | Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-7. PMID: 8078761
- Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. | Molloy, MP., et al. 1998. Electrophoresis. 19: 837-44. PMID: 9629924
- Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. | Herbert, BR., et al. 1998. Electrophoresis. 19: 845-51. PMID: 9629925
Utilisation :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of GENIUS™ Nuclease is 1.3×106 ~ 1.5×106 unit/mg protein.
Formulation :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
État Physique :
Liquid
Dérivé de :
Recombinant protein expressed in Escherichia coli
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
WGK Allemagne :
1
Numéro MDL :
MFCD00131010
Numéro CE :
231-791-2
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Region
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SAMPLE Certificate of Analysis (COA)
GENIUS™ Nuclease | CAS 9025-65-4 SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
Envoyer
SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-202391
Lot Number:SAMPLE
Product Name:GENIUS™ Nuclease
Native Source:Recombinant protein expressed in Escherichia coli
Test | Specification | Results |
---|---|---|
Activity | Biological activity: > 250U/µL | 625 U/µL |
Appearance | Clear, no visible particles | Complies |
Purity (SDS-PAGE) | > 95% | Complies |
Specific Activity | ≥ 1.0 x106 unit/mg | Complies |
Endotoxin by LAL | <1 EU/µg | Complies |
Protease | Not detectable | Complies |
Sterility | < 0.1 CFU/ml | Complies |
TEST CONDITIONS
Exp. Date: 12/14/2027
Informations pour la commande
Nom du produit | Ref. Catalogue | COND. | Prix HT | QTÉ | Favoris | |
GENIUS™ Nuclease, 25 KU | sc-202391 | 25 KU | .00 | |||
GENIUS™ Nuclease, 500 KU | sc-202391A | 500 KU | 1.00 |