Goat anti-Adenosine A2a Receptor
Referentie EB07079
Formaat : 100ug
Merk : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB07079 | Goat Anti-Adenosine A2a Receptor Antibody | Pep-ELISA, WB, IF | 100µg specific antibody in 200µl | Human, Mouse, Macaque |
Target Protein
Principal Names: ADORA2A, adenosine A2a receptor, HGNC:263, ADORA2, RDC8, hA2aR, adenosine A2 receptor, adenosine receptor subtype A2aOfficial Symbol: ADORA2A
Accession Number(s): NP_000666.2
Human GeneID(s): 135
Immunogen
Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:8000.Western blot: This antibody has been successfully used in Western blot on Macaque: van der Putten C et al. (2009) PMID: 19494284. This antibody has been successfully used in Western blot on Mouse: He X et al. (2013) PMID: 23712033, and Zhao L et al. (2015) PMID: 26171727.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10µg/ml.
Species Reactivity
Tested: Human, Mouse, MacaqueExpected from sequence similarity: Human
Specific References
This antibody has been successfully used in WB on Mouse:
Zhao L, Liu YW, Yang T, Gan L, Yang N, Dai SS, He F.
The mutual regulation between miR-214 and A2AR signaling plays an important role in inflammatory response.
Cell Signal. 2015 Oct;27(10):2026-34.
PMID: 26171727
This antibody has been successfully used in the WB on Mouse:
He X, Hu JL, Li J, Zhao L, Zhang Y, Zeng YJ, Dai SS, He FT.
A feedback loop in PPARγ-adenosine A2A receptor signaling inhibits inflammation and attenuates lung damages in a mouse model of LPS-induced acute lung injury.
Cell Signal. 2013 Sep;25(9):1913-23.
PMID: 23712033
This antibody has been successfully used in WB on Macaque:
van der Putten C, Zuiderwijk-Sick EA, van Straalen L, de Geus ED, Boven LA, Kondova I, IJzerman AP, Bajramovic JJ.
Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia.
J Immunol. 2009 Jun 15;182(12):7603-12.
PMID: 19494284
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol