H-MESO-1A
Merk : CLS Cell Lines Service
H-MESO-1A Cells
General information
Description | The H-MESO-1A cell line is derived from human mesothelioma, a type of cancer that originates in the mesothelial cells lining the lungs, abdomen, or heart. This cell line is particularly valuable for research focused on understanding the pathophysiology of mesothelioma and the development of therapeutic strategies. Mesothelioma is often linked to asbestos exposure, and the H-MESO-1A cells can be used to study the molecular mechanisms underlying asbestos-induced carcinogenesis. H-MESO-1A cells exhibit the characteristic features of mesothelioma, including aggressive growth and resistance to conventional chemotherapy. They are utilized in preclinical studies to evaluate the efficacy of novel drugs, gene therapy approaches, and immunotherapy strategies. Researchers use this cell line to investigate the genetic and epigenetic alterations associated with mesothelioma, as well as to identify potential biomarkers for early diagnosis and prognosis. The H-MESO-1A cell line is an essential tool in the advancement of mesothelioma research and the quest for effective treatments. |
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Organism | Human |
Tissue | Lung |
Disease | Pleural Mesothelioma |
Synonyms | H-Meso-1A, H-Meso 1A, H-Meso1A, HMeso01A, HMESO1A, HMeso1A |
Characteristics
Age | 35 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | H-MESO-1A (Cytion catalog number 300187) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 negative |
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Tumorigenic | Yes, in nude mice |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | Every 5 to 7 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,y CSF1PO: 11,12 D13S317: 11 D16S539: 12 D5S818: 10,12 D7S820: 12 TH01: 3.3 TPOX: 8 vWA: 17 D3S1358: 14 D21S11: 30,33.2 D18S51: 14,20 Penta E: 7,11 Penta D: 11,13 D8S1179: 10 FGA: 23 |
HLA alleles | A*: 02:01:01 B*: 13:02:01, 44:02:01 C*: 06:02:01, 07:04:01 DRB1*: 07:01:01, 13:01:01 DQA1*: 01:03:01, 02:01:01 DQB1*: 02:02:01, 06:03:01 DPB1*: 03:01:01, 20:01:01 E: 01:01, 01:03 |