IL6 ELISA Kit (Human)

Referentie OKEH00140

Formaat : 96Wells

Merk : Aviva Systems Biology

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Telefoonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL6 ELISA Kit (Human) (OKEH00140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL6 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
IL6 Microplate96 Wells (12 x 8 Well strips)
IL6 Lyophilized Standard2
100X Biotinylated IL6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity9.8 pg/mL
SpecificityNatural and recombinant Human Interleukin-6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL6
Gene Full Nameinterleukin 6
Alias SymbolsB-cell differentiation factor;B-cell stimulatory factor 2;BSF2;BSF-2;CDF;CTL differentiation factor;HGF;HSF;hybridoma growth factor;IFNB2;IFN-beta-2;IL-6;interferon beta-2;interleukin BSF-2;interleukin-6.
NCBI Gene Id3569
Protein NameP05231
Description of TargetInterleukin-6
Uniprot IDhttps://www.uniprot.org/uniprot/P05231
Protein Accession #NP_000591
Nucleotide Accession #NM_000600
Protein Size (# AA)212
Molecular Weight24 kDa

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OKEH00052
 96T