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> IL6 ELISA Kit (Human)

IL6 ELISA Kit (Human)

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Referentie OKEH00140

Formaat : 96Wells

Merk : Aviva Systems Biology

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Telefoonnummer : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL6 ELISA Kit (Human) (OKEH00140) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL6 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
IL6 Microplate96 Wells (12 x 8 Well strips)
IL6 Lyophilized Standard2
100X Biotinylated IL6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity9.8 pg/mL
SpecificityNatural and recombinant Human Interleukin-6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL6
Gene Full Nameinterleukin 6
Alias SymbolsB-cell differentiation factor;B-cell stimulatory factor 2;BSF2;BSF-2;CDF;CTL differentiation factor;HGF;HSF;hybridoma growth factor;IFNB2;IFN-beta-2;IL-6;interferon beta-2;interleukin BSF-2;interleukin-6.
NCBI Gene Id3569
Protein NameP05231
Description of TargetInterleukin-6
Uniprot IDhttps://www.uniprot.org/uniprot/P05231
Protein Accession #NP_000591
Nucleotide Accession #NM_000600
Protein Size (# AA)212
Molecular Weight24 kDa
Biological pathways
Name# of Products
Acute-phase response209
Cellular response to hydrogen peroxide43
Defense response to Gram-negative bacterium93
Defense response to Gram-positive bacterium56
Defense response to virus284
Endocrine pancreas development246
Glucagon secretion22
Hepatic immune response18
Humoral immune response159
Interleukin-6-mediated signaling pathway33
Monocyte chemotaxis65
Negative regulation of apoptotic process538
Negative regulation of cell proliferation554
Negative regulation of chemokine biosynthetic process28
Negative regulation of collagen biosynthetic process20
Negative regulation of fat cell differentiation103
Negative regulation of lipid storage119
Neuron projection development76
Neutrophil apoptosis32
Neutrophil mediated immunity28
Platelet activation875
Positive regulation of acute inflammatory response31
Positive regulation of anti-apoptosis118
Positive regulation of B cell activation24
Positive regulation of chemokine production128
Positive regulation of immunoglobulin secretion60
Positive regulation of interleukin-6 production149
Positive regulation of leukocyte chemotaxis30
Positive regulation of osteoblast differentiation109
Positive regulation of peptidyl-serine phosphorylation168
Positive regulation of sequence-specific DNA binding transcription factor activity174
Positive regulation of smooth muscle cell proliferation123
Positive regulation of STAT protein import into nucleus20
Positive regulation of T cell proliferation211
Positive regulation of transcription from RNA polymerase II promoter1210
Positive regulation of translation71
Positive regulation of tyrosine phosphorylation of Stat3 protein101
Regulation of angiogenesis60
Regulation of vascular endothelial growth factor production22
Response to glucocorticoid stimulus111
Response to peptidoglycan18

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