LDH1/2 H4/H3M Isozyme Rabbit Polyclonal Antibody
Referentie AP21335AF-N
Formaat : 10mg
Product Data | |
Application | ELISA, ID, IF, IP, R, WB |
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Application | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Recommended Working Dilutions: Non-precipitating antibody-binding techniques: Up to 1/500. |
Reactivity | Human |
Antibody Host | Rabbit |
Clonality | Polyclonal |
Immunogen | L-Lactic Dehydrogenase, LDH-1(H4)/LDH-2(H3M) isoenzyme, is isolated and purified from Human placenta. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Specificity | The antibody recognizes LDH-1(H4)/LDH-2(H3M) isoenzyme, is isolated and purified from Human placenta. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Buffer | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
Concentration | lot specific |
Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. If a slight precipitation occurs upon storage, this should be removed by centrifugation Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Reference Data |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.