Lentivirus Precipitation Reagent

Referentie VC150

Formaat : 500mL

Merk : Alstem

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Lentivirus Precipitation Solution

Highlights:
  • Easy-to-use: simply mix
  • No ultracentrifugation required
  • Easily scale up for large volumes
  • Up to 100 fold concentration increase
  • Cost effective spin protocol for efficient viral concentration
  • Non-toxic: safe for all cell lines, including ES cells
Description:

Lentivirus Precipitation Solution is a mixture of polymers optimized for the precipitation of lentiviral particles. It provides a simple, fast and highly efficient method for concentrating lentiviral particles. The protocol involve mixing your lentiviral supernatant with the Lentivirus Precipitation Solution, incubate for a short period, and spin the mixture in a standard centrifuge. You'll increase your lentivirus titer by up to 100 fold as quick as in 4 hrs, and obtain excellent recoveries without ultracentrifugation. The Lentivirus Precipitation Solution is a 5x solution.

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Specifications:
Product Name Lentivirus Precipitation Solution
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at 4 °C. This product is stable for 6 months when stored as directed.

Quality Control

Each lot of Lentivirus Precipitation Solution is tested for sterility and successful precipitation of Lentiviral particles.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

  1. J.Y. Lee et al., YAP-independent mechanotransduction drives breast cancer progression. Nat Commun 10, (2019).

  2. E. Mailer et al., The autophagy protein ATG9A promotes HIV-1 infectivity. Retrovirology 16 (2019).
  3. V. Achuthan et al., Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA Integration. Cell Host Microbe 24, (2018).
  4. B. Adamson et al., Approaches to maximize sgRNA-barcode coupling in Perturb-seq screens. bioRxiv (2018).
  5. R. Hsieh et al., CDK19 is a Regulator of Triple-Negative Breast Cancer Growth. bioRxiv (2018).
  6. E. Shifrut et al., Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Cell 175, (2018).
  7. P. Younan et al., Role of Transmembrane Protein 16F in the Incorporation of Phosphatidylserine Into Budding Ebola Virus Virions. J Infect Dis 218, (2018).
  8. M. Puray-Chavez et al., Multiplex single-cell visualization of nucleic acids and protein during HIV infection. Nat Commun 8, (2017).

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VB100
 1mL