Images
Flow Cytometry
Application

Flow Cytometry

Thymocytes from a young chicken were isolated and incubated with MCAM monoclonal antibody, clone C264. The cells were then stained with goat anti-mouse IgG2b-FITC and analyzed on a FACScan™ flow cytometer (BDB, San Jose, CA).

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against native MCAM.

    Immunogen

    Native purified chicken MCAM.

    Host

    Mouse

    Reactivity

    Chicken

    Specificity

    Chicken MCAM, Mr 98 KDa (protein core =84 KDa).

    Form

    Liquid

    Conjugation

    FITC

    Isotype

    IgG2b, kappa

    Recommend Usage

    Flow Cytometry (1 ug/106 cells)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (0.09% sodium azide)

    Storage Instruction

    Store in the dark at 4°C. Do not freeze.
    Avoid prolonged exposure to light.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry (Frozen sections)

    Immunoprecipitation

    Flow Cytometry

    Thymocytes from a young chicken were isolated and incubated with MCAM monoclonal antibody, clone C264. The cells were then stained with goat anti-mouse IgG2b-FITC and analyzed on a FACScan™ flow cytometer (BDB, San Jose, CA).
  • Gene Info — MCAM

    Entrez GeneID

    448832

    Gene Name

    MCAM

    Gene Alias

    -

    Gene Description

    melanoma cell adhesion molecule

    Gene Ontology

    Hyperlink

    Other Designations

    HEMCAM|gicerin

  • Publication Reference