Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.

Mdivi-1 [338967-87-6]

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Referentie HY-15886-5mg

Formaat : 5mg

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Telefoonnummer : +1 850 650 7790

Merk : MedChemExpress

Neem contact op met een lokale distributeur :

Telefoonnummer : +1 850 650 7790

Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

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Mdivi-1 Chemical Structure

CAS No. : 338967-87-6

This product is a controlled substance and not for sale in your territory.

Based on 149 publication(s) in Google Scholar

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Vet Microbiol. 2023 May.; S.aureus-induced reduction of mitochondrial proteins is blocked by Mdivi-1 (20 μM; 2 h) in BMECs.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Theranostics. 2019 Feb 28;9(6):1698-1713. [Abstract]; Western blotting of SDs and markers of podocyte dedifferentiation with the treatment of PA , BBR + PA and Mdivi1+PA.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232. [Abstract]; HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 48 h. Apoptosis-related proteins expression level is detected by western blotting. β-actin was used as a loading control.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232. [Abstract]; HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 24 h. Mitochondrial colocalization with LC3 is detected by immunofluorescence.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Brain Res. 2019 May 15;1711:68-76. [Abstract]; Levels of CytC in cytosol (Cyto-CytC), CytC in mitochondria (Mito- CytC) and cleaved caspase-3 in hippocampus are determined by western blot with the treatment of sham, HIR, MDV or FK506.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: J Pineal Res. 2018 Sep;65(2):e12491. [Abstract]; Inhibition of mitochondrial fission with mdivi-1 or Drp1 siRNA suppresses hyperglycemia-induced oxidative stress. Protein expression of Nox4.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Transl Res. 2018 Apr;194:68-78. [Abstract]; Representative western blot analysis of pDrp1-616 and total Drp1 protein expression in the hippocampus (left). Tubulin is used as the loading control. Protein expressions are quantified by western blot band intensity (right).

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2017 Nov;112:336-349. [Abstract]; Mdivi-1 administration reduces NF-κB-dependent inflammation. A. Western blot assay and quantitative analysis of Cyto-p65 (A) and Nec-p65 (B) expression. ß-actin and H3 are used as internal controls of cytosolic and nuclear subfractions, respectively.

: Mdivi-1 purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2017 Sep;295:116-124. [Abstract]; Mdivi-1 treatment inhibits the expression of DRP1, alleviates ROS and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of DRP1.

Description

Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

In Vitro

Mdivi-1 inhibits Dnm1 GTPase activity in a dose-dependent manner, with an estimated EC₅₀ of 1-10 μM. Mdivi-1 increases the apparent K_0.5 for GTP, lowers the apparent V_max for GTP hydrolysis, and causes an increase in the Hill coefficient observed for GTP in the Dnm1 GTP hydrolysis reaction^[1]. Cells treated with mdivi-1 display decreased cytochrome c release and a reduced rate of phosphatidylserine exposure on their surface following apoptosis induction, consistent with an inhibition of apoptosis and with previous studies using other strategies to compromise DRP1 activity^[2]. Mdivi-1 results in apoptotic cell death in ischemic retina^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The mitochondrial division DRP, Dnm1, is the target of mdivi-1 in vivo. Mdivi-1 quantitatively blocks GMPPCP- dependent Dnm1 self-assembly in a concentration range similar to its effects on mitochondrial division in vivo^[1]. Mdivi-1 (50 mg/kg, i.p.) significantly decreases GFAP protein expression in the normal mouse retina^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

353.22

Formule

C₁₅H₁₀Cl₂N₂O₂S

CAS No.

338967-87-6

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1N(C2=C(Cl)C=C(Cl)C(OC)=C2)C(S)=NC3=C1C=CC=C3

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 160 mg/mL (452.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8311 mL	14.1555 mL	28.3110 mL
5 mM	0.5662 mL	2.8311 mL	5.6622 mL
10 mM	0.2831 mL	1.4155 mL	2.8311 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité
Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

This protocol yields a clear solution of ≥ 4 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 2.5 mg/mL (7.08 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 17% Solutol HS-15 in Saline
Solubility: 10 mg/mL (28.31 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureté et documentation

Purity: 99.73%

Select Batch:

Fiche technique (281 KB) SDS (394 KB)

COA (253 KB) LCMS (138 KB)

Instruction de manipulation (2659 KB)

Références

[1]. Cassidy-Stone A, et al. Chemical inhibition of the mitochondrial division dynamin reveals its role in Bax/Bak-dependent mitochondrial outer membrane permeabilization. Dev Cell. 2008 Feb;14(2):193-204. [Content Brief]

[2]. Tanaka A, et al. A chemical inhibitor of DRP1 uncouples mitochondrial fission and apoptosis. Mol Cell. 2008 Feb 29;29(4):409-10. [Content Brief]

[3]. Park SW, et al. A selective inhibitor of drp1, mdivi-1, increases retinal ganglion cell survival in acute ischemic mouse retina. Invest Ophthalmol Vis Sci. 2011 Apr 27;52(5):2837-43. [Content Brief]

Essai de kinase ^[1]	All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity. MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Test cellulaire ^[1]	YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system. MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Références	[1]. Cassidy-Stone A, et al. Chemical inhibition of the mitochondrial division dynamin reveals its role in Bax/Bak-dependent mitochondrial outer membrane permeabilization. Dev Cell. 2008 Feb;14(2):193-204. [Content Brief] [2]. Tanaka A, et al. A chemical inhibitor of DRP1 uncouples mitochondrial fission and apoptosis. Mol Cell. 2008 Feb 29;29(4):409-10. [Content Brief] [3]. Park SW, et al. A selective inhibitor of drp1, mdivi-1, increases retinal ganglion cell survival in acute ischemic mouse retina. Invest Ophthalmol Vis Sci. 2011 Apr 27;52(5):2837-43. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8311 mL	14.1555 mL	28.3110 mL	70.7774 mL
	5 mM	0.5662 mL	2.8311 mL	5.6622 mL	14.1555 mL
	10 mM	0.2831 mL	1.4155 mL	2.8311 mL	7.0777 mL
	15 mM	0.1887 mL	0.9437 mL	1.8874 mL	4.7185 mL
	20 mM	0.1416 mL	0.7078 mL	1.4155 mL	3.5389 mL
	25 mM	0.1132 mL	0.5662 mL	1.1324 mL	2.8311 mL
	30 mM	0.0944 mL	0.4718 mL	0.9437 mL	2.3592 mL
	40 mM	0.0708 mL	0.3539 mL	0.7078 mL	1.7694 mL
	50 mM	0.0566 mL	0.2831 mL	0.5662 mL	1.4155 mL
	60 mM	0.0472 mL	0.2359 mL	0.4718 mL	1.1796 mL
	80 mM	0.0354 mL	0.1769 mL	0.3539 mL	0.8847 mL
	100 mM	0.0283 mL	0.1416 mL	0.2831 mL	0.7078 mL

Mdivi-1 [338967-87-6]

Referentie HY-15886-5mg

Neem contact op met een lokale distributeur :

Merk : MedChemExpress

Neem contact op met een lokale distributeur :

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Mdivi-1 Related Classifications

Keywords:

Misschien heeft u ook interesse in de volgende producten:

Mdivi-1 [338967-87-6]

Referentie HY-15886-5mg

Neem contact op met een lokale distributeur :

Merk : MedChemExpress

Neem contact op met een lokale distributeur :

Mdivi-1 Related Antibodies

Calculateur de molarité

Calculateur de dilution

Complete Stock Solution Preparation Table

Mdivi-1 Related Classifications

Keywords:

Misschien heeft u ook interesse in de volgende producten: