D-dimer is a protein formed by two D fragments of the fibrin protein joined by a cross-link. D-dimer is one of several fibrin degradation product (FDP) formed by the degradation of a blood clot by fibrinolysis. It is used in the diagnosis of the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis.

This kit is designed for the quantitative measurement of Fibrinogen D-Dimer protein in Monkey Serum, plasma and other biological fluids.

Introduction	D-dimer (or D dimer) is a fibrin degradation product (or FDP), a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis. It is so named because it contains two D fragments of the fibrin protein joined by a cross-link. Ddimers are not normally present in human blood plasma, except when the coagulation system has been activated, for instance because of the presence of thrombosis or disseminated intravascular coagulation. As a result, D-dimer concentration may be determined by a blood test to help diagnose thrombosis and has become an important test performed in patients with suspected thrombotic disorders.
Target	D-Dimer
Reactivity	Monkey
Tested Applications	ELISA
Recommended dilutions	Optimal dilutions/concentrations should be determined by the end user.
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability	The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
Test Range	62.5 ng/ml - 4000 ng/ml
Sensitivity	37.5 ng/ml
Standard Form	Lyophilized
Detection Method	Colorimetric
Assay Type	Sandwich
Assay Data	Quantitative
Sample Type	Plasma.
Kit Components	The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. Pre-coated 96-Well Microplate Standard Standard Diluent Buffer Wash Buffer Detection Reagent A Detection Reagent B Diluent A Diluent B TMB Substrate Stop Solution Plate Sealer
Material Required But Not Provided	37°C incubator Multi and single channel pipettes and sterile pipette tips Squirt bottle or automated microplate washer 1.5 ml tubes Distilled water Absorbent filter papers 100 ml and 1 liter graduated cylinders Microplate reader (wavelength: 450 nm) ELISA Shaker
Assay Procedure	This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product. 1) Set standard, test samples and control wells. 2) Aliquot 100 µl of diluted standard into the standard wells. 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well. 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C. 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C. 6) Wash 3 times. 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C. 8) Wash 5 times. 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C. 10) Aliquot 50 µl of Stop Solution. 11) Measure the OD at 450 nm.
Assay Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Monkey Fibrinogen D-Dimer protein were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Monkey Fibrinogen D-Dimer protein were tested on 3 different plates, 8 replicates in each plate. CV (%) = (Standard Deviation / mean) × 100 Intra-Assay: CV<10% Inter-Assay: CV<10%
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with	Antibody

Research Articles on D-Dimer