Mouse Anti-Human CD106-UNLB
Referentie 9510-01
Formaat : 0.1mg
Merk : Southern Biotech
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Telefoonnummer : +1 850 650 7790
| Clone | 1.G11B1 |
|---|---|
| Isotype | Mouse IgG1κ |
| Isotype Control | Mouse IgG1-UNLB (15H6) |
| Specificity | Human/Porcine CD106 |
| Alternative Names | VCAM-1, INCAM-110, vascular cell adhesion molecule-1 |
| Description | CD106, also known as INCAM-110, is a 110 kDa vascular adhesion cell adhesion molecule-1 (VCAM-1) that is member of the immunoglobulin superfamily. CD106 is expressed predominantly on cytokine-activated vascular endothelium but has also been identified on interfollicular dendritic cells, some macrophages, and bone marrow stromal cells. Endothelial CD106 binds the integrins α4β1 (CD49d/CD29, VLA-4) and α4β7 and contributes to extravasation of lymphocytes, monocytes, basophils, and eosinophils (but not neutrophils) from blood vessels, particularly at sites of inflammation. Unlike the β2 integrins, the CD106-VLA-4 interaction can mediate both the initial tethering and rolling of lymphocytes on endothelium as well as their subsequent arrest and firm adhesion. CD106 expressed on non-vascular tissues has been implicated in the interaction of hematopoietic progenitors with bone marrow stromal cells, B cell binding to follicular dendritic cells, costimulation of T cells, and embryonic development. The monoclonal antibody 1.G11B1 inhibits in vitro binding of lymphocytes and monocytes to VCAM-1 on stimulated endothelium. |
| Conjugate | UNLB (Unconjugated) |
| Buffer Formulation | Borate buffered saline, pH 8.2 |
| Clonality | Monoclonal |
| Concentration | 0.1 mg/mL |
| Volume | 1.0 mL |
| Recommended Storage | 2-8°C |
| Applications | Flow Cytometry – Quality tested 9-15 Immunohistochemistry-Frozen Sections – Reported in literature 3 Immunocytochemistry – Reported in literature 4,5 Immunoprecipitation – Reported in literature 1 Western Blot-Non-Reducing – Reported in literature 2 ELISA – Reported in literature 1,6-8 Blocking – Reported in literature 1 Adhesion – Reported in literature 1 |
| RRID Number | AB_2796822 |
| Gene ID | 7412 (Human) 396925 (Porcine) |
| Gene ID Symbol | VCAM1 (Human) VCAM1 (Porcine) |
| Gene ID Aliases | CD106; INCAM-100 |
| UniProt ID | P19320 (Human |
| UniProt Name | VCAM1_HUMAN (Human) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Thornhill MH, Wellicome SM, Mahiouz DL, Lanchbury JS, Kyan-Aung U, Haskard DO. Tumor necrosis factor combines with IL-4 or IFN-γ to selectively enhance endothelial cell adhesiveness for T cells. The contribution of vascular cell adhesion molecule-1-dependent and -independent binding mechanisms. J Immunol. 1991;146:592-8. (ELISA, IP, Adhesion, Block)
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- 8. Lester EA, Babensee JE. Proinflammatory phenotype of endothelial cells after coculture with biomaterial-treated blood cells. J Biomed Mater Res A. 2003;64:397-410. (ELISA)
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- 15. Moscoso I, Centano A, López E, Rodriguez-Barbosa JI, Santamarina I, Filgueira P, et al. Differentiation "in vitro" of primary and immortalized porcine mesenchymal stem cells into cardiomyocytes for cell transplantation. Transplant Proc. 2005;37:481-2. (FC, Porcine Reactivity)