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Mouse bFGF

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Referentie 600182

Formaat : 0.05mg

Merk : Abbiotec

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Recombinant Active Protein
Cat. No. 
600182
Unit 
0.05 mg
Description 

Basic fibroblast growth factor (bFGF or Heparin-binding growth factor 2) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. Several different forms of the protein exist, ranging from 18-24 kDa in size due to the use of alternative start sites within the FGF-2 gene. It has a 55 percent amino acid residue identity to FGF-1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor. Recombinant Mouse basic FGF (FGF-2) produced in E. coli is a non-glycosylated single polypeptide chain containing 145 amino acids with a MW of 16,320 Da.

Each vial contains 50 ug lyophilized protein. Reconstitute with 0.5 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Alternate Names 
Heparin-binding growth factor 2; HBGF-2; Basic fibroblast growth factor; BFGF; FGF2; FGF-2
Accession No.  
P15655
MW 
16320 Da
Sequence 
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Source 
E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Purity 
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Application Notes 
The ED50, calculated by the dose-dependent proliferation of BALB/3T3 cells is 0.1 ng/ml.
Storage 
Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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For research use only, not for diagnostic or therapeutic procedures.